• dnaLIMS Login |
  • Research Core Facilities |
  • Follow @MUGenomicsCore
10x Genomics Single Cell Flow Cell Calculator (updated)

10x Genomics Single Cell Calculator


Calculator will help determine the correct flow cell selection to achieve necessary read depth on single cell projects.

The sequencing parameters and number of cycles required for the different single cell applications can be found on the core's 10x Genomic page.



Number of Samples            

Target Cell Count                

Targeted Reads Per Cell      

Total Reads Required      

                    Flow Cell Use Ratio*
NovaSeq X Lane (2-digit precision)     
NovaSeq X Flow Cell (2-digit precision)     
Estimated sequencing cost                      $


Assumptions used in calculations:

  • NovaSeq X flow cell (lane) = 1.25 billion paired reads
  • NovaSeq X flow cell = 10 billion paired reads