Newsletter Contents
Technology Updates
• Visium HD 3' Spatial GEX Assay
• GEM-X On-Chip Multiplexing (OCM)

Service Highlights
• PacBio and ONT Long Read Sequncing
Upcoming Seminar
• 10x Genomic/ONT Seminar

Technology Updates
Expanded Visium Spatial Gene Expression Capture
The Visium HD 3' Spatial Gene Expression assay is designed to analyze poly(A) RNA in tissue sections derived from fresh frozen (FF) tissue samples.  Collection of the RNA is mediated by the Visium CytAssist instrument, where tissue and slide are  brought into proximity with one another. Tissue on the tissue slide is permeabilized, allowing for the release of poly(A) RNA and subsequent capture by the spatially-barcoded oligonucleotides present on the Visium HD Slide surface.
WT Probe Assay Features
• Probe based chemistry for human and mouse
• Broad range of tissue compatibility: FFPE, fresh frozen, and fixed frozen
• 18,000+ protein coding genes
• 6.5 x 6.5 mm (2 Capture Areas per slide)
WT 3’ Assay Features
•3' poly(A) capture
•Species agnostic
•Starts with fresh frozen tissue
•Whole transcriptome
•6.5 x 6.5 mm (2 Capture Areas per slide)
Learn more at 10xGenomics - 10x Genomics Visium Products
GEM-X On-Chip Multiplexing (OCM)
The GEM-X Universal 3'v4 and 5'v3 Gene Expression 4-plex assays provide a scalable microfluidic platform for on-chip multiplexing (OCM) of up to eight samples (two sets of up to four samples each).
Sample multiplexing is accomplished by partitioning up to four samples from each multiplexing set with corresponding gel beads (each containing a unique list of barcodes). A pool of GEMs is generated in a single recovery well for each set. The GEM pool in each recovery well is used to generate cDNA, where DNA derived from a cell shares a common barcode.
Advantages
• Compatible with Universal 3’ GEX and Universal 5’ GEX Assays
• No tagging samples before chip loading, simplifying the process and reducing preparation time and sample loss.
• Reduces the per sample reagent cost
• Maximum count of 5,000 cells per sample (vs. 20,000 standard)
Learn more at 10xGenomics - What is Sample Multiplexing?
Service Highlight
PacBio and Oxford Nanopore Long Read Sequncing
Long read sequencing typically provide read lengths >15 Kbases and help resolve challenging genomic regions such as complex structural variants. Long reads also provide better genome assemblies overcoming the difficulties in mapping repetitive regions or regions of high GC content that lack coverage with short-read technologies.
The GTC has an outsourcing arrangement with the Genome Technology Access Center at the McDonnell Genome Institute at Washington University. Long read sequencing services are available using either a PacBio Revio or Oxford Nanopore Promethion24. Core staff will handle all submission arrangements, shipment of samples, and manage payments for MU researchers. Data will be made available through the core's data distribution pipeline and stored according to GTC data retention policies.

Contact the Genomics Core (mugenonomicscore@missouri.edu), to learn more about this service.
Upcoming Seminar
Join us to learn how synergizing the resolution of 10x Genomics single-cell and spatial profiling with the depth of Oxford Nanopore long-read sequencing is revolutionizing our understanding of biology.
Following the presentation, representatives will be available for one-on-one discussions between 2:00 p.m. – 3:30 p.m.
RSVP
Genomics Technology Core | University of Missouri
Life Science Center, Rm 216
1201 Rollins StreetColumbia, MO 65211
Phone: 573.882.0428
Email: mugenomicscore@missouri.edu
Web: mugenomicscore.missouri.edu/
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