The Illumina DNA prep is a commercially available kit that uses a tagmentation process to fragment genomic DNA and add adapter sequences using Bead-Linked Transposomes. The streamlined protocol delivers consistent insert sizes and uniform coverage regardless of DNA input amount or genome size. The method can be used for a wide range of applications, from mammalian genomes to amplicons and microbial genomes.
Unique dual indexes are available for multiplexing of up to 384 samples. Genomic DNA input is 1 ng - 500 ng.
Core staff are available to assist with experimental design and to answer questions related to next-generation sequencing technology. Arrangements for a meeting to discuss new or existing projects can be made by sending an email to Nathan Bivens, Genomics Core director.
All projects must be initiated with a quote before samples are submitted. Send a quote request to the general core email mugenomicscore@missouri.edu.
The Genomic Calculator may be used to estimate the amount of sequence required for a project.
General Considerations:
- Submit samples only after paperwork has been completed.
- Clearly label tubes. Names must match those given on the project information form.
- Low binding microcentrifuge tubes (0.5 or 1.5 mL) are preferred for storing high quality nucleic acids.
Submission Requirements:
| Input Range | Submission Concentration |
Recommended Buffer |
Special Instructions |
| 1-500 ng | 25 - 200 ng/μl genomic DNA | 10mM Tris (pH 8.5) | An image of 100 ng of genomic DNA following agarose gel electrophoresis and staining will be provided to demonstrate gDNA integrity. |
| Sequence Depth/Coverage* | Read Type | Index Type | Read Length | ||
| 30x coverage | Paired end | Unique dual index | 150 bases | ||
* coverage recommended by MUGTC for mammalian genome; increased coverage may be desired for some studies