The versatility of the NGS library prep allows any DNA fragment to be sequenced. The core provides library prep services with NEBNext Ultra II DNA Libary Prep Kit from New England Biolabs to prepare library from cDNA, amplicons, chromatin immunoprecipitated DNA, and other DNA. Contact the Genomics Core to learn how libraries can be prepared from your DNA samples.
The Illumina NovaSeq X Plus instrument will provide a read length of 150 bases. Amplicon libraries requiring longer reads can be sequenced on the MiSeq platform for a maximum read length of 300 bases. Core staff can assist with the identification of the optimal read length for your project.
Unique dual indexes are available for multiplexing of up to 96 samples. DNA input is 100 pg - 100 ng.
Core staff are available to assist with experimental design and to answer questions related to next-generation sequencing technology. Arrangements for a meeting to discuss new or existing projects can be made by sending an email to Nathan Bivens, Genomics Core director.
All projects must be initiated with a quote before samples are submitted. Send a quote request to the general core email mugenomicscore@missouri.edu.
General Considerations:
- Submit samples only after paperwork has been completed.
- Clearly label tubes. Names must match those given on the project information form.
- Low binding microcentrifuge tubes (0.5 or 1.5 mL) are preferred for storing high quality nucleic acids.
Submission Requirements:
| Input Range | Submission Concentration |
Recommended Buffer |
Special Instructions |
| 100 pg - 100 ng | 1 - 20 ng/μl DNA | 10mM Tris (pH 8.5) |
| Sequence Depth/Coverage* | Read Type | Index Type | Read Length | ||
| determine by experimental goals | Paired end | Unique dual index | 150 - 300 bases | ||