The Illumina Stranded mRNA Prep is a commercially available kit from Illumina used to prepare stranded RNA-Seq libraries from messenger RNA (mRNA). Oligo(dT) magnetic beads capture and enrich the mRNA molecules containing poly A tails. Total RNA integrity is evaluated for each sample prior to library construction by processing on a Fragment Analyser to view the rRNA profile.
Unique dual-indexes are available for multiplexing of up to 384 samples. Total RNA input is 25 ng - 1000 ng.
Core staff are available to assist with experimental design and to answer questions related to next-generation sequencing technology. Arrangements for a meeting to discuss new or existing projects can be made by sending an email to Nathan Bivens, Genomics Core director.
All projects must be initiated with a quote before samples are submitted. Send a quote request to the general core email mugenomicscore@missouri.edu.
The RNA-Seq Calculator may be used to estimate the amount of sequence required for a project.
General Considerations:
- Submit samples only after paperwork has been completed.
- Clearly label tubes. Names must match those given on the project information form.
- Low binding microcentrifuge tubes (0.5 or 1.5 mL) are preferred for storing high quality nucleic acids.
Submission Requirements:
| Input Range | Submission Concentration |
Recommended Buffer |
Special Instructions |
| 25-1000 ng | 50 - 150 ng/μl total RNA | nuclease free water | Provide sample source and extraction methods used. DNase treatment strongly recommended. |
| Sequence Depth/Coverage* | Read Type | Index Type | Read Length | ||
| 50 million reads per sample | Paired end | unique dual-index | 100 bases | ||
* minimum read count recommended by MUGTC; increased sequencing depth may be desired for some studies