The Genomics Technology Core has provided next generation sequencing services to the UM system since 2008. The GTC maintain and operate several Illumina sequencing platforms; NovaSeq X Plus and MiSeq (3) instruments.
The NovaSeq X Plus offers Illumina's most advanced sequencer to date while dramatically lowering costs. The release of the 25B flow cell introduced a 2.5-fold increase in output over that of the previous NovaSeq 6000. The new XLEAP-SBS chemistry delivers increased speed and greater fidelity. Each flow cell contains 8 independent lanes, which can be used to load up to 8 separate library pools.
NovaSeq X Plus
| Flow Cell | Read Length | # of Lanes | Paired Reads/Lane (M) | Data Output (Gb/lane) |
| 10B | PE150 | 8 | 1,250 | 375 |
| 25B | PE150 | 8 | 3,250 | 975 |
MiSeq
| Flow Cell | Read Length | Paired Reads/Lane (M) | Data Output (Gb/lane) |
| MiSeq PE150 v2 | PE150 | 12-15 M | 4.5-5.1 Gb |
| MiSeq PE250 v2 | PE250 | 12-15 M | 7.5-8.5 Gb |
| MiSeq PE300 v3 | PE300 | 22-25 M | 13.2-15 Gb |