Sanger Sequencing Services | Troubleshooting Guide



Sequencing Guides


QIAGEN Guide to Template Purification and DNA Sequencing (2nd Edition)             PDF icon
DNA Sequencing by Capillary Electrophoresis Applied Biosystems Chemistry Guide (Second Edition)             PDF icon


Reference Articles


Adams P.S., et. al. (1998) An analysis of techniques used to improve the accuracy of automated DNA sequencing of a GC-rich template: Results form the 2nd ABF DNA sequence research group study. Journal of Biomolecular Techniques 9(4):9-18.

Bellis, G. De, et. al. (1992) A More Stringent Choice of Primers Can Improve the Performace of Flourescent Automated DNA Sequencers. Biotechniques 13(6):892-897.

Buck, G.A., et. al. (1999) Design strategies and performance of custom DNA sequencing primers. BioTechniques 27(3):528-536.

Etokebe, G.E. and Anne Spurkland (2000) Method for avoiding PCR-inhibiting contaminants when eluting DNA from polyacrylamide gels. BioTechniques 29(4):694-696.

Grayburn, W.S. and T.L. Sims (1998) Anchored oligo(dT) primers for automated dye terminator DNA sequencing. BioTechniques 25(3):340-346.

Kumari, D. and K. Usdin (1999) Sequencing errors in reactions using labeled terminators. BioTechniques 27(4):648-649.

Leonard, J.T. et. al. (1998) Preparation of PCR products for DNA sequencing. BioTechniques 24(2):314-317.

Liao, Ji and Zhiyuan Gong (1997) Sequencing of 3' cDNA clones using anchored oligo(dt) primers. BioTechniques 23(3):368-370.

Mahlknecht, U., et. al. (1999) Sequencing of genomic DNA. BioTechniques 27(3):406-408.

Ripoll, J., et. al. (2000) Technique for cloning and sequencing the ends of bacterial artificial chromosome inserts. BioTechniques 29(2):271-276.