The GEM-X Flex assay provides a scalable solution to measure gene expression from up to 384 fixed samples within a single GEM reaction. A whole transcriptome probe panel targets the mRNA. Probe panels now available for human, mouse and rat. Up to 20k cells per sample can be targeted. The Flex Apex assay increases throughput dramatically with a 96-well format for sample handling. The fixation protocol allows samples to be stored for up to 1 year without losing data quality.
Key features of the service:
- Joint consultation available with Genomics Core and Bioinformatics Core.
- Cell counting with K2 Cellometer and cell suspension loading.
- Chromium X instrunment to process cells with latest GEM-X reagents.
- 10x library construction and rigorous library QC.
- Sequence generation performed on a NovaSeq X Plus platform providing economical sequencing and project scalability.
- Biosafety level 2 containment available for cell collection.
Additional documentation and resources are available on the 10x Genomics support site
A meeting is required with core staff to initiate a single cell project. The meeting will discuss experimental goals as well as project turnaround times, collection scheduling, cell concentration requirements, and cell/nuclei quality requirements. Arrangements for a meeting to discuss a project can be made by sending an email to Nathan Bivens, Genomics Core director.
All projects must be initiated with a quote, and quote accepted, before a collection can be scheduled. .
The 10x Genomics Single Cell Calculator may be used to estimate the amount of sequence required for a project.
General Considerations:
- Submit cells in Eppendorf DNA Lo Bind Tubes, 2.0 ml (Cat. No. 022431048)
- Must minimize presence of cellular aggregates, dead cells, non-cellular nucleic acids and reverse transcription inhibitors.
- On the scheduled day of submission, core staff should be notified 1 hour prior to cells/nuclei being delived. It is best practice to provide periodic updates to core staff on the day of collection.
Submission Requirements:
| Cell/Nuclei Concentration | Volume | Required Buffer | Special Instructions |
| minimum of 300k cells for probe hybridization | 30 - 100 ul | Diluted Nuclei Buffer *provided in kit |
Review the 10x protocol on how to fix cells and nuclei. |
| Sequence Depth/Coverage* | Read Type | Index Type | Read Length | ||
| minimum 10,000 read pairs/cell | Paired end | unique dual-index | 150 bases | ||
* minimum read count recommended by MUGTC; increased sequencing depth may be desired for some studies