The 10x Genomics Chromium System provides for the massive partitioning and barcoding of single cells/nuclei using ~3,600,000 unique barcodes with the GEM-X chemistry. At the heart of the Chromium System is 10x GemCode™ Technology. Partitioning events occur on a microfluidic chip in the presence of barcoded gel beads and oil to create GEMs (Gel Bead in EMulsion). As many as 20,000 cells per sample are encapsulated in nano-liter scale GEMs. Final amplification and library construction is performed in bulk after GEMs are broken. Resulting libraries are compatible with Illumina sequencing platforms.

All sequencing is generated on an Illumina NovaSeq X Plus. The NovaSeq X platform is particularly useful for differential gene expression studies due to the high data yields, multiplexing capabilities, and high data quality. Quality control and quality assurance metrics are incorporated into each standard operating procedure to monitor performance of each service provided.