The Visium Spatial Gene Expression Slide allows for the detection of gene expression across intact fresh frozen tissue. Superimposing the gene expression information upon an image of the tissue allows researchers to understand the spatial organization of cell types.
The Visium Spatial Services are provided in collaboration with the Advanced Light Microscopy Core and the Bioinformatics and Analytics Core.
Additional documentation and resources are available on the 10x Genomics support site
A meeting is required with core staff to initiate a single cell project. The meeting will discuss experimental goals as well as project turnaround times, collection scheduling, cell concentration requirements, and cell/nuclei quality requirements. Arrangements for a meeting to discuss a project can be made by sending an email to Nathan Bivens, Genomics Core director.
All projects must be initiated with a quote, and quote accepted, before a collection can be scheduled. .
The 10x Spatial Calculator may be used to estimate the amount of sequence required for a project.
The initial step in a Visium Spatial protocol is the cryosectioning and placement of the tissue on the Visium slide. The 10x Genomics Tissue Preparation Guide provides guidance:
- Best practices for handling tissue samples and Visium Spatial slides before and after cryosectioning.
- Freezing and embedding tissue samples prior to cryosectioning.
- Cryosectioning of tissue samples and placement of sections on Visium Spatial slides.
Support and services for tissue section, staining and imaging is available through the Advanced Light Microscopy Core.
| Sequence Depth/Coverage | Read Type | Index Type | Read Length | ||
| 50,000 reads per spot | Paired end | unique dual-index | 100 bases | ||