RNA Sequencing (RNA-Seq) has become a widely used method to evaluate the differential expression of genes across the whole trascriptome in a variety of biological conditions and organisms. The direct sequencing of the RNA transcript also provides detection of splice variants, transcript isoforms and single nucleotide variants. The core provides several different methods for RNA-Seq library construction to accommodate varying sample types, including methods for low input and partially degraded samples, as well as options for polyA enrichment or ribosomal reduction.

Small RNA sequencing is also available enabling analysis of miRNA and other small RNA species for small RNA discovery and profiling.

All sequencing is generated on an Illumina NovaSeq X Plus. The NovaSeq X platform is particularly useful for differential gene expression studies due to the high data yields, multiplexing capabilities, and high data quality. Quality control and quality assurance metrics are incorporated into each standard operating procedure to monitor performance of each service provided.